spot colocalization function (Oxford Instruments)
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spot colocalization function
Spot Colocalization Function, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44244 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spot+colocalization+function/Imaris/pmc09909199-44-21-25
Average 99 stars, based on 44244 article reviews
Spot Colocalization Function, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44244 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spot+colocalization+function/Imaris/pmc09909199-44-21-25
Average 99 stars, based on 44244 article reviews
spot colocalization function - by Bioz Stars,
2026-09
99/100 stars
Images
1) Product Images from "Nuclear translocation of spike mRNA and protein is a novel feature of SARS-CoV-2"
Article Title: Nuclear translocation of spike mRNA and protein is a novel feature of SARS-CoV-2
Journal: Frontiers in Microbiology
doi: 10.3389/fmicb.2023.1073789
Figure Legend Snippet: The nuclear translocation of S protein and S mRNA includes both the outer surface and inside of the nucleus. Separate slides (see ) were imaged under a Leica Stellaris confocal microscope (Leica) using a 63x oil objective. The images were then deconvolved using Huygen Essential deconvolution software (Scientific Volume Imaging). Using the surface rendering function of an image processing IMARIS software. (A) S mRNA (red) on the nuclear surface (top) and inside the nucleus (bottom). White arrows indicate S protein on the nuclear surface (top image) or inside the nucleus (bottom image). (B) S protein (green) on the nuclear surface (top image) and inside the nucleus (bottom image). White arrows indicate S protein on the nuclear surface (top image) or inside the nucleus (bottom image). (C) The total distribution of S mRNA and S protein in the cells. The data were obtained by combining multiple images from an independent experiment. (D) The total colocalization between S mRNA and S protein in the cells. The data were obtained by combining multiple images from an independent experiment.
Techniques Used: Translocation Assay, Microscopy, Software, Imaging
Figure Legend Snippet: Colocalization between S mRNA and S protein inside infected cells. The images (see ) were analyzed by using the surface rendering and colocalization features of IMARIS. S protein and S mRNA distribution and colocalization in the cytoplasm (top panel), on the nuclear surface (middle panel) and inside the nucleus (bottom panel). The specific region of colocalization is indicated by a white spot. Scale bar 0.5 μm.
Techniques Used: Infection
Related Articles
Software:Article Title: Nuclear translocation of spike mRNA and protein is a novel feature of SARS-CoV-2. Article Snippet: .. To confirm the physical apposition between S mRNA and the nucleus by comparing their distributions in fluorescent images, we used the Article Title: Nuclear translocation of spike mRNA and protein is a novel feature of SARS-CoV-2 Article Snippet: .. To confirm the physical apposition between S mRNA and the nucleus by comparing their distributions in fluorescent images, we used the Article Title: Nuclear translocation of spike mRNA and protein is a novel pathogenic feature of SARS-CoV-2 Article Snippet: .. To confirm the physical apposition between S mRNA and the nucleus by comparing their distributions in fluorescent images, we used the Double Immunostaining:Article Title: Mapping the connectivity of serotonin transporter immunoreactive axons to excitatory and inhibitory neurochemical synapses in the mouse limbic brain Article Snippet: .. Fig. 7 3D-reconstruction and quantification of inhibitory 5-HTergic triads throughout the limbic brain. a Micrograph representing a double immunostaining of the presynaptic marker synaptophysin ( blue ) and the inhibitory postsynaptic marker gephyrin ( red ) in the BLA. b Use of the Article Title: Mapping the connectivity of serotonin transporter immunoreactive axons to excitatory and inhibitory neurochemical synapses in the mouse limbic brain. Article Snippet: .. In this study, we have adapted a semi-automated method combining multi-step immunolabeling and high-resolution confocal imaging to reconstruct in 3D a total of 195.43 mm of SERT-immunoreactive axons and map the distribution of synaptophysin-immunoreactive boutons forming synaptic triads with excitatory or inhibitory neurochemical Fig. 5 3D-reconstruction and quantification of excitatory 5-HTergic triads throughout the limbic brain. a Micrograph representing double immunostaining of the presynaptic marker synaptophysin (blue) and the excitatory postsynaptic marker PSD95 (purple) in the BLA. b The use of the Article Title: Mapping the connectivity of serotonin transporter immunoreactive axons to excitatory and inhibitory neurochemical synapses in the mouse limbic brain. Article Snippet: .. Among the brain regions analyzed, we found that the highest proportions of SYNSERT? boutons engaged in triads were in the BLA and CeA of the amygdala, reaching 61 Fig. 7 3D-reconstruction and quantification of inhibitory 5-HTergic triads throughout the limbic brain. a Micrograph representing a double immunostaining of the presynaptic marker synaptophysin (blue) and the inhibitory postsynaptic marker gephyrin (red) in the BLA. b Use of the Marker:Article Title: Mapping the connectivity of serotonin transporter immunoreactive axons to excitatory and inhibitory neurochemical synapses in the mouse limbic brain Article Snippet: .. Fig. 7 3D-reconstruction and quantification of inhibitory 5-HTergic triads throughout the limbic brain. a Micrograph representing a double immunostaining of the presynaptic marker synaptophysin ( blue ) and the inhibitory postsynaptic marker gephyrin ( red ) in the BLA. b Use of the Article Title: Mapping the connectivity of serotonin transporter immunoreactive axons to excitatory and inhibitory neurochemical synapses in the mouse limbic brain. Article Snippet: .. In this study, we have adapted a semi-automated method combining multi-step immunolabeling and high-resolution confocal imaging to reconstruct in 3D a total of 195.43 mm of SERT-immunoreactive axons and map the distribution of synaptophysin-immunoreactive boutons forming synaptic triads with excitatory or inhibitory neurochemical Fig. 5 3D-reconstruction and quantification of excitatory 5-HTergic triads throughout the limbic brain. a Micrograph representing double immunostaining of the presynaptic marker synaptophysin (blue) and the excitatory postsynaptic marker PSD95 (purple) in the BLA. b The use of the Article Title: Mapping the connectivity of serotonin transporter immunoreactive axons to excitatory and inhibitory neurochemical synapses in the mouse limbic brain. Article Snippet: .. Among the brain regions analyzed, we found that the highest proportions of SYNSERT? boutons engaged in triads were in the BLA and CeA of the amygdala, reaching 61 Fig. 7 3D-reconstruction and quantification of inhibitory 5-HTergic triads throughout the limbic brain. a Micrograph representing a double immunostaining of the presynaptic marker synaptophysin (blue) and the inhibitory postsynaptic marker gephyrin (red) in the BLA. b Use of the Immunolabeling:Article Title: Mapping the connectivity of serotonin transporter immunoreactive axons to excitatory and inhibitory neurochemical synapses in the mouse limbic brain. Article Snippet: .. In this study, we have adapted a semi-automated method combining multi-step immunolabeling and high-resolution confocal imaging to reconstruct in 3D a total of 195.43 mm of SERT-immunoreactive axons and map the distribution of synaptophysin-immunoreactive boutons forming synaptic triads with excitatory or inhibitory neurochemical Fig. 5 3D-reconstruction and quantification of excitatory 5-HTergic triads throughout the limbic brain. a Micrograph representing double immunostaining of the presynaptic marker synaptophysin (blue) and the excitatory postsynaptic marker PSD95 (purple) in the BLA. b The use of the Imaging:Article Title: Mapping the connectivity of serotonin transporter immunoreactive axons to excitatory and inhibitory neurochemical synapses in the mouse limbic brain. Article Snippet: .. In this study, we have adapted a semi-automated method combining multi-step immunolabeling and high-resolution confocal imaging to reconstruct in 3D a total of 195.43 mm of SERT-immunoreactive axons and map the distribution of synaptophysin-immunoreactive boutons forming synaptic triads with excitatory or inhibitory neurochemical Fig. 5 3D-reconstruction and quantification of excitatory 5-HTergic triads throughout the limbic brain. a Micrograph representing double immunostaining of the presynaptic marker synaptophysin (blue) and the excitatory postsynaptic marker PSD95 (purple) in the BLA. b The use of the |